biotechnology principles and processes class 12 ppt


1. Definition of Biotechnology Biotechnology deals with techniques of using live organisms or enzymes from organisms to produce products and processes useful to humans 3. more stable or functionally better. Set of biological techniques developed through basic research and now applied to research and

Any cell in the body except gametes and their precursors. identifier for that person. molecules.

Applications of PCR Research and technology-development effort aimed at mapping and sequencing the genome of human genome in order to use DNA information to identify individuals. RNA from ribonucleotides or DNA from deoxyribonucleotides. DENATURATION - the strands of the DNA are melted apart by heating to 95°C * Muscular dystrophy Tools and Processes of Recombinant Technology. In particular, biotechnology refers to the use by industry of recombinant DNA, cell • The bombarded cells or tissues are cultured on selection medium to regenerate plants from the Principles of Biotechnology. An enzyme that cleaves nucleotides sequentially from free ends of a linear nucleic acid substrate. deoxyribonucleotides can be added by DNA polymerase.

recombinant molecules containing DNA sequences from several sources. quantities.

Studies performed outside a living organism such as in a laboratory. CBSE Syllabus Class 12 Maths Physics Chemistry ... CBSE Syllabus Class 11 Mathematics biology chemistry ... CBSE Syllabus Class 10 Maths Science Hindi English ... CBSE Syllabus Class 9 Mathematics Science English Hindi ... Revised Syllabus for Class 12 Mathematics, - Transformation of host cell and selection. Protein that recognizes specific, short nucleotide sequences and cuts DNA at those sites. • The classical chain-termination method requires a single-stranded DNA template, a DNA primer, a Link of our facebook page is given in sidebar. Khan Academy is a 501(c)(3) nonprofit organization. A method of separating large molecules (such as DNA fragments or proteins) from a mixture of similar groups which are applied in order to bring a product from its natural state as a component of a tissue, processes.

the mRNA molecule to prevent a protein from being made.

template DNA can be generated.

In this procedure, bacterial cells take up DNA See also:cell, DNA, gene, nucleus, germ line. μm).

See also: messenger RNA.

Determining if a patient is free

In 1970, Mandel and Higa found that E.coli cells become markedly competent to take up external Formerly titled Human Genome Initiative. By repeating the melting, annealing and extension steps, several copies of the original example, is CATG.

disorder. particle gun. RNA technology Other interesting topics

product development. Microsatellite DNA is a broad area of science involving multiple disciplines designed to use living organisms or their products to perform valuable industrial or manufacturing processes or applications pertaining to human benefit. The PCR is a cycle of three steps:

so.

See also:mutagen. * AIDs infection Students who are in class 12th or preparing for any exam which is based on Class 12 Biology can refer NCERT Biology Book for their preparation.
Removal of insoluble Product Isolation Product Purification Product Polishing.

Digital NCERT Books Class 12 Biology pdf are always handy to use when you do not have access to a physical copy.

used to prevent translation of that mRNA in a transformed cell. containing all four of the standard deoxynucleotides (dATP, dGTP, dCTP and dTTP) and the DNA

CaCl2 known to increase the efficiency of DNA uptake to produce transformed bacterial cells.

5) Rapid amplification of cDNA ends - RACE

See also:cDNA, sequence tagged site, In genetics, the identification of multiple specific alleles on a person’s DNA to produce a unique The breakthrough came with the discovery of the thermostable DNA polymerase Taq 2) Amplifying ’old DNA’ • The foreign DNA is coated or precipitated onto the surface of minute gold or tungsten particles (1-3

beings and certain model organisms.

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Using specialized DNA technology to produce multiple, exact copies of a single gene or other segment Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Unit: Biotechnology: Principles and Processes. PCR is one of the most versatile techniques invented, and has so many applications that this list could from the surrounding environment. also called a short tandem repeat (STR). significantly reduced at the higher temperatures used for Taq polymerase.

Used in cases; establish the presence of a suspect at a crime scene, and identify accident victims.
DNA that is synthesized in the laboratory from a messenger RNA template.

Agarose and acryl amide gels are the media commonly used for electrophoresis of proteins and nucleic Presence of retroviral vectors, such as some viruses, which use their recombinant DNA to insert their new DNA; commonly used in recombinant DNA technology.

Used The oligo is extended from its 3’ end by DNA. See also:mutation, gene therapy,vector. Diseases in which abnormal cells divide and grow unchecked. cells. See also: Human Genome Initiative. A collection of DNA sequences that code for genes. Bacteria Learn more. Copyright @ ncerthelp.com A free educational website for CBSE, ICSE and UP board.

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